Journal: JBMR Plus

Article Title: Establishment and characterization of a human pre-osteocyte cell line: hOsteo4-E9

doi: 10.1093/jbmrpl/ziaf163

Figure Lengend Snippet: RNA-sequencing data revealed that hOsteo4-E9 cells not only share some similarity with MLO-Y4 cells, but also have distinct transcription profiles in response to FSS. (A) PCA plot of gene expression variability of human hOsteo4-E9, murine MLO-Y4, and murine long bone-derived osteocytes; (B) Venn diagram of detected genes in hOsteo4-E9 and MLO-Y4 cells; (C) Venn diagram of detected genes in 4 experimental groups, that is, E9_F, E9_S, Y4_F, and Y4_S; (D-F) top 15 of KEGG enriched pathways in all detected genes, hOsteo4-E9 specific and MLO-Y4 specific genes; (G) clustering heat map highlighted the variations of FA-associated gene expression patterns between 4 experimental groups; (H-M) qPCR validation of gene expression of integrin αν, integrin α5, integrin α3, integrin β5, integrin β7, and EMP1 in 4 experimental groups. N = 3 for each group. Results are expressed as mean ± SD. n.s. p > .05; * p < .05; ** p < .01; *** p < .001.

Article Snippet: Next, 1 μg of total RNA was reverse-transcribed into cDNA using an RNA reverse transcription kit (Cat# 11141ES60, Yeasen, Shanghai, China).

Techniques: RNA Sequencing, Gene Expression, Derivative Assay, Biomarker Discovery